What will the future of medicine look like? The Nanobiomedicine Laboratory is creating the future medicine with nanobiotechnology and bio-electro-mechanical fusion technologies to realize precise and tiny diagnostic devices as well as therapeutic systems. Another name for our lab is the “BioDr.” (Bioinspired Diagnostic-regenerative technology) lab, providing a mission to “Be like a doctor curing disease with biological technology.” The BioDr. Lab is interested in preventive medicine as a future medical realm and we expect stimulation-based modulation will be one of the core technologies for health maintenance. In this regard, we focus on the development of effective physical stimulation systems toward aging recovery and rejuvenation. We study biological mechanisms responding to various cellular and whole-body stimuli and find optimal stimulation condition for the functional improvement of aging cells and tissues. The research activities of BioDr. Lab can be categorized by (1) Aging organ-on-a-chip based on 3D bioprinting, (2) Stimulation-based rejuvenation, (3) Aged stem cell activation and (4) Electroceuticals.
BioDr. Lab is developing novel aging study tools and studying cellular responses based on physical simulations. The organs and tissues we focus on include skin, skeletal muscle, nerve and fat. By utilizing BioMEMS (microelectromechanical systems), bioelectronics, microfluidics and 3D bioprinting technologies, both implantable devices and cell stimulation biochips have been constructed.
(1) Aging organ-on-a-chip (AOC)
It is very important to mimic the in vivo microenvironment of aged organs and tissues for aging recovery. Based on BioMEMS and 3D bioprinting technology, we create models of aged tissue including skin, muscle, neurons, etc. This system, the aging organ-on-chip (AOC), can by itself provide a valuable platform to clearly elucidate the mechanisms and modes of action for the aging process and recovery. There is great demand among the R&D departments of many cosmetic and pharmaceutical companies for these technologies, including AOC and artificial tissue fabrication. Moreover, various aging platforms including C. elegans, yeast, mammalian and plant cell models are also under active investigation and development.
(2) Stimulation based rejuvenation
Aging cells intrinsically exhibit long doubling times: hence, are very rare. There are several kinds of physical stimulation sources including plasma, ultrasound, electric and magnetic fields, which comprises unlimited stimulation conditions. We have therefore created novel lab-on-a-chips for high throughput stimulation screening (HTSS), in order to analyze cellular responses on a massively parallel scale. BioDr. Lab is currently developing physical stimulation-based high throughput screening lab-on-a-chip platform to improve the function of aged primary skeletal muscle cells.
(3) Aged stem cell activation
Tissue homeostasis and repair in aged organisms depends on aged stem cells which constitute a very limited population of undifferentiated long-lived cells in whole-body tissues. To increase the viability and functional performance of aging organisms, BioDr. Lab focuses on the activation of these aged stem cells staged in senescence, in particular studying the mechanism of stem cell reactivation and their response to key environmental stimuli. Currently, we are interested in reactivation of aged skeletal muscle stem cells and their response and molecular changes to such stimuli.
(4) IoT based bioelectronics
As our world has been turned into the 4th industrial revolution, the capability of information processing has now entered into nearly everything. Given that intelligent devices are getting smaller by virtues of nanotechnology, aging related nanobiosensors can be implemented in a wearable form-factor. BioDr. Lab is also paying attention to electroceuticals, implantable electronic devices designed to modulate biological systems for pharmaceutical purposes. We are developing a novel remote electroceutical to repair degeneration in the peripheral nervous system.
- Kim MS, Jo S, Park JT, Shin HY, Kim SS, Gurel O, Park SC, Method to purify and analyze heterogeneous senescent cell populations using a microfluidic filter with uniform fluidic profile, Anal Chem., 87, 9584, 2015
- Park JM, Kim MS, Moon HS, Yoo CE, Park D, Kim YJ, Han KY, Lee JY, Oh JH, Kim SS, Park WY, Lee WY, Huh N, Fully automated circulating tumor cell isolation platform with large-volume capacity based on lab-on-a-disc, Anal Chem, 86, 3735, 2014/li>
- Kim MS, Kim J, Lee W, Cho SJ, Oh JM, Lee JY, Baek S, Kim YJ, Sim TS, Lee HJ, Jung GE, Kim SI, Park JM, Oh JH, Gurel O, Lee SS, Lee JG, A trachea-inspired bifurcated microfilter capturing viable circulating tumor cells via altered biophysical properties as measured by atomic force microscopy, Small, 9, 3103, 2013/li>
- Lee HJ, Oh JH, Oh JM, Park JM, Lee JG, Kim MS, Kim YJ, Kang HJ, Jeong J, Kim SI, Lee SS, Choi JW, Huh N, Efficient isolation and accurate in situ analysis of circulating tumor cells using detachable beads and a high-pore-density filter, Angew Chem Int Ed Engl, 52, 8337, 2013/li>
- Kim MS, Sim TS, Kim YJ, Kim SS, Jeong H, Park JM, Moon HS, Kim SI, Gurel O, Lee SS, Lee JG, Park JC, SSA-MOA: a novel CTC isolation platform using selective size amplification (SSA) and a multi-obstacle architecture (MOA) filter, Lab Chip, 12, 2874, 2012/li>
- Park GS, Kwon H, Kwak DW, Park SY, Kim MS, Lee JH, Han H, Heo S, Li XS, Lee JH, Kim YH, Lee JG, Yang W, Cho HY, Kim SK, Kim K, Full surface embedding of gold clusters on silicon nanowires for efficient capture and photothermal therapy of circulating tumor cells, Nano Lett, 12, 1638, 2012/li>
- Kim MS, Kwon S, Kim T, Lee ES, Park JK, Quantitative proteomic profiling of breast cancers using a multiplexed microfluidic platform for immunohistochemistry and immunocytochemistry, Biomaterials, 32, 1396, 2011/li>
- Kim MS, Kim T, Kong SY, Kwon S, Bae CY, Choi J, Kim CH, Lee ES, Park JK, Breast cancer diagnosis using a microfluidic multiplexed immunohistochemistry platform, PLoS One, 5, e10441, 2010/li>