|Degree||Michign State University, PhD.|
How plants know when and how to die?
Leaves undergo developmental and physiological changes during their lifespan, ending with senescence and death. Leaf senescence is an important developmental phase and considerably impacts crop yields. During senescence, leaves change their role from nutrient-accumulating organs through carbon fixation and nutrient uptake to nutrient-exporting organs, resulting in nutrient recycling to developing organs.
Leaf senescence is a part of the plant developmental process but can be triggered by environmental changes that are integrated into the developmental aging program. This integrated senescence response determines the pattern of leaf senescence and contributes to improved survival in a given ecological niche.
The focus of the research is aimed at increasing our knowledge of leaf senescence at the molecular and biochemical levels. The elucidation of their mechanisms of regulation will present an insight into the complex processes that control this key developmental process and allow future manipulation of senescence for agronomic benefit.
A.Systems Biology of Arabidopsis leaf senescence
Our overall goal is to gain insights into system-level understanding of leaf senescence at molecular, cellular, intercellular levels. To investigate age-dependent changes in multiple transcriptomes over the leaf lifespan, we performed directional total RNA-seq and small RNA-seq analysis of Arabidopsis leaves collected at 14 and 7 time points, respectively, encompassing the entire lifespan. These data provide a set of interesting and important hypotheses.
B. Temporal dynamics of RNA-RNA, RNA-Protein, RNA-DNA networks along leaf life history and senescence
- Studying the long noncoding RNAs-mediated regulatory mechanisms using various functional analyses
- Understanding the importance of regulatory IncRNAs on plant fitness.
- Understanding molecular basis of RNA-DNA-protein interaction in leaf development.
C. Spatiotemporal design of the hierarchical biological clocks in Arabidopsis
- Study on Spatiotemporal transition of biological clock along leaf development
- Identification of genetic machineries maintaining the coordination and designing spatiotemporal transition of biological clock
- Predication of leaf cell fate by monitoring cellular clock
D. Computational Study of Leaf Senescence through a Phenomic Approach
Leaf senescence is influenced by its life history, comprising a series of developmental and physiological experiences. Exploration of the biological principles underlying leaf lifespan and senescence requires a schema to trace leaf phenotypes, based on the interaction of genetic and environmental factors. We developed a new approach and concept that will facilitate systemic biological understanding of leaf lifespan and senescence, utilizing the phenome high-throughput investigator (PHI) with a single-leaf-basis phenotyping platform. Our pilot tests showed empirical evidence for the feasibility of PHI for quantitative measurement of leaf senescence responses and improved performance in order to dissect the progression of senescence triggered by different senescence-inducing factors as well as genetic mutations. Such an establishment enables new perspectives to be proposed, which will be challenged for enhancing our fundamental understanding on the complex process of leaf senescence. We further envision that integration of phenomic data with other multi-omics data obtained from transcriptomic, proteomic, and metabolic studies will enable us to address the underlying principles of senescence, passing through different layers of information from molecule to organism.
- Investigation of association among phenomic responses, lifespan, and various environmental cues in various accessions through PHI
(Plant High-throughput Investigator) system
– Dissection of molecular networks in accessions with distinct lifespan history