Pyung Ok Lim
|Lab||Plant Senescence Laboratory|
How plants know when and how to die?
Leaves undergo developmental and physiological changes during their lifespan, ending with senescence and death. Leaf senescence is an important developmental phase and considerably impacts crop yields. During senescence, leaves change their role from nutrient-accumulating organs through carbon fixation and nutrient uptake to nutrient-exporting organs, resulting in nutrient recycling to developing organs.
Leaf senescence is a part of the plant developmental process but can be triggered by environmental changes that are integrated into the developmental aging program. This integrated senescence response determines the pattern of leaf senescence and contributes to improved survival in a given ecological niche.
The focus of the research is aimed at increasing our knowledge of leaf senescence at the molecular and biochemical levels. The elucidation of their mechanisms of regulation will present an insight into the complex processes that control this key developmental process and allow future manipulation of senescence for agronomic benefit.
A. Systems Biology of Arabidopsis leaf senescence
- Multi-dimensional leaf lifespan transcriptome analysis of Arabidopsis
- Studying regulatory networks delineating functional coordination of correlated expression transition along leaf aging
- Understanding the inter-organellar coordination along leaf aging
B. Temporal dynamics of RNA-RNA, RNA-Protein, RNA-DNA networks along leaf life history and senescence
- Studying the long noncoding RNAs-mediated regulatory mechanisms using various functional analyses
- Understanding the importance of regulatory IncRNAs on plant fitness.
- Understanding the molecular basis of RNA-DNA-protein interaction in leaf development.
C. Spatiotemporal design of the hierarchical biological clocks in Arabidopsis
- Study on Spatiotemporal transition of biological clock along leaf development
- Identification of genetic machineries maintaining the coordination and designing spatiotemporal transition of biological clock
- Predication of leaf cell fate by monitoring cellular clock
D. Computational Study of Leaf Senescence through a Phenomic Approach
- Investigation of association among phenomic responses, lifespan, and various environmental cues in various accessions through PHI (Plant High-throughput Investigator) system
- Dissection of molecular networks in accessions with distinct lifespan history
- Woo HR, Kim HJ, Lim PO*, Nam HG* (2019) Leaf senescence: Systems and dynamics Aspects Annual review of plant biology *Co-corresponding author
- Lyu JI, kim JH, Chu H, Taylor MA, Jung S, Baek SH, Woo HR, Lim PO*, Kim J* (2019) Natural allelic variation of GVS1 confers diversity in the regulation of leaf senescence in Arabidopsis. New Phytologist 221:2320-2334 *Co-corresponding author
- Kim HJ, Park JH, Kim JK, Kim JJ, Hong SH, Kim J, Kim J, Woo HR, Hyeon C, Lim PO*, Hwang D*, and Nam HG* (2018) A NAC troika directs aging by regulatory inversion of age-evolving. Proc Natl Acad Sci U S A 115: E4930–4939. *Co-corresponding author
- Km J., Park SJ, Lee IH, Chu H , Penfold CA, Kim JH , Buchanan-Wollaston, Nam HG, Woo HR, and Lim PO (2018) Comparative transcriptome analysis in Arabidopsis ein2/ore3 and ahk3/ore12 mutants during dark-induced leaf senescence, J Exp Bot. 69:3023-3036
- Lee SY, Jeong HB, Lee SC, Lee JW, Kim SJ, Park JW, Woo HR, Lim PO, An GH, Nam HG and Hwang DH (2017) Molecular bases for differential aging programs between flag and second leaves during grain-filling in rice, Scientific Reports, 7:8792
- Lyu JI, Baek SH, Jung S, Chu H, Nam HG, Kim J, and Lim PO (2017) High-throughput and computational study of leaf senescence through a phenomic approach, Frontiers in Plant Science, 8:250
- Woo HR, Koo HJ, Kim J, Yang JO, Lee IW, Jun J, Choi SH, Park SJ, Kang B, Kim YW, Phee BK, Kim JH, Seo C, Park C, Kim SC, Park S, Lee B, Lee S, Hwang D, Nam HG and Lim PO (2016) Programming of plant leaf senescence with temporal and inter-organellar coordination of transcriptome in Arabidopsis, Plant Physiology, 171:452-467.